Journalartikel - Artikel | 2014 | Peer reviewed

Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats

Autor(en)

Dealtry, Simone ; Ding, Guo-Chun ; Weichelt, Viola ; Dunon, Vincent ; Schlüter, Andreas ; Carla Martini, Maria ; Florencia Del Papa, Maria ; Lagares, Antonio ; Amos, Gregory Charles Auton ; Wellington, Elizabeth Margaret Helen ; Gaze, William Hugo ; Sipkema, Detmer ; Sjoling, Sara ; Springael, Dirk ; Heuer, Holger ; van Elsas, Jan Dirk ; Thomas, Christopher ; Smalla, Kornelia

Normierter Zeitschriftentitel

PLOS ONE

DOI

10.1371/journal.pone.0089922

ISSN

1932-6203

PubMed-ID

24587126

URL

https://pub.uni-bielefeld.de/record/2669237

Web of Science-ID

000331880700116

Weitere Details

Sprache

englisch

Darstellungsform

Text

Band

Artikelnummer

e89922

Ausgabe

IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC- DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots'' of plasmids potentially carrying catabolic genes.

Zitierstile

Dealtry S, Ding G-C, Weichelt V, et al. Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE. 2014;9(2): e89922.

In die Zwischenablage einfügen

Dealtry, S., Ding, G. - C., Weichelt, V., Dunon, V., Schlüter, A., Carla Martini, M., Florencia Del Papa, M., et al. (2014). Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE, 9(2), e89922. doi:10.1371/journal.pone.0089922

In die Zwischenablage einfügen

Dealtry, Simone, Ding, Guo-Chun, Weichelt, Viola, Dunon, Vincent, Schlüter, Andreas, Carla Martini, Maria, Florencia Del Papa, Maria, et al. 2014. “Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats”. PLoS ONE 9 (2): e89922.

In die Zwischenablage einfügen

Dealtry, S., Ding, G. - C., Weichelt, V., Dunon, V., Schlüter, A., Carla Martini, M., Florencia Del Papa, M., Lagares, A., Amos, G. C. A., Wellington, E. M. H., et al. (2014). Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE 9:e89922.

In die Zwischenablage einfügen

Dealtry, S., et al., 2014. Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE, 9(2): e89922.

In die Zwischenablage einfügen

S. Dealtry, et al., “Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats”, PLoS ONE, vol. 9, 2014, : e89922.

In die Zwischenablage einfügen

Dealtry, S., Ding, G.-C., Weichelt, V., Dunon, V., Schlüter, A., Carla Martini, M., Florencia Del Papa, M., Lagares, A., Amos, G.C.A., Wellington, E.M.H., Gaze, W.H., Sipkema, D., Sjoling, S., Springael, D., Heuer, H., van Elsas, J.D., Thomas, C., Smalla, K.: Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE. 9, : e89922 (2014).

In die Zwischenablage einfügen

Dealtry, Simone, Ding, Guo-Chun, Weichelt, Viola, Dunon, Vincent, Schlüter, Andreas, Carla Martini, Maria, Florencia Del Papa, Maria, Lagares, Antonio, Amos, Gregory Charles Auton, Wellington, Elizabeth Margaret Helen, Gaze, William Hugo, Sipkema, Detmer, Sjoling, Sara, Springael, Dirk, Heuer, Holger, van Elsas, Jan Dirk, Thomas, Christopher, and Smalla, Kornelia. “Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats”. PLoS ONE 9.2 (2014): e89922.

In die Zwischenablage einfügen

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In die Zwischenablage einfügen

Dealtry, S., et al., 2014. Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats. PLoS ONE, 9(2): e89922.

In die Zwischenablage einfügen

S. Dealtry, et al., “Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats”, PLoS ONE, vol. 9, 2014, : e89922.

In die Zwischenablage einfügen

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