Journalartikel - Artikel | 2015 | Peer reviewed

Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates

Autor(en)

Wibberg, Daniel ; Rupp, Oliver ; Blom, Jochen ; Jelonek, Lukas ; Kröber, Magdalena ; Verwaaijen, Bart ; Goesmann, Alexander ; Albaum, Stefan ; Grosch, Rita ; Pühler, Alfred ; Schlüter, Andreas

Normierter Zeitschriftentitel

PLOS ONE

DOI

10.1371/journal.pone.0144769

PubMed-ID

26690577

URN

urn:nbn:de:0070-pub-29012970

URL

https://pub.uni-bielefeld.de/record/2901297

Web of Science-ID

000367092300015

Weitere Details

Sprache

englisch

Darstellungsform

Text

Band

Artikelnummer

e0144769

Ausgabe

Link zum Volltext (intern)

https://pub.uni-bielefeld.de/record/2901297

DDC

570

Rhizoctonia solani, a soil-born plant pathogenic basidiomycetous fungus, affects various economically important agricultural and horticultural crops. The draft genome sequence for the R. solani AG1-IB isolate 7/3/14 as well as a corresponding transcriptome dataset (Expressed Sequence Tags-ESTs) were established previously. Development of a specific R. solani AG1-IB gene model based on GMAP transcript mapping within the eukaryotic gene prediction platform AUGUSTUS allowed detection of new genes and provided insights into the gene structure of this fungus. In total, 12,616 genes were recognized in the genome of the AG1-IB isolate. Analysis of predicted genes by means of different bioinformatics tools revealed new genes whose products potentially are involved in degradation of plant cell wall components, melanin formation and synthesis of secondary metabolites. Comparative genome analyses between members of different R. solani anastomosis groups, namely AG1-IA, AG3 and AG8 and the newly annotated R. solani AG1-IB genome were performed within the comparative genomics platform EDGAR. It appeared that only 21 to 28% of all genes encoded in the draft genomes of the different strains were identified as core genes. Based on Average Nucleotide Identity (ANI) and Average Amino-acid Identity (AAI) analyses, considerable sequence differences between isolates representing different anastomosis groups were identified. However, R. solani isolates form a distinct cluster in relation to other fungi of the phylum Basidiomycota. The isolate representing AG1-IB encodes significant more genes featuring predictable functions in secondary metabolite production compared to other completely sequenced R. solani strains. The newly established R. solani AG1-IB 7/3/14 gene layout now provides a reliable basis for post-genomics studies.

Zitierstile

Wibberg D, Rupp O, Blom J, et al. Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates. Plos One. 2015;10(12): e0144769.

In die Zwischenablage einfügen

Wibberg, D., Rupp, O., Blom, J., Jelonek, L., Kröber, M., Verwaaijen, B., Goesmann, A., et al. (2015). Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates. Plos One, 10(12), e0144769. doi:10.1371/journal.pone.0144769

In die Zwischenablage einfügen

Wibberg, Daniel, Rupp, Oliver, Blom, Jochen, Jelonek, Lukas, Kröber, Magdalena, Verwaaijen, Bart, Goesmann, Alexander, et al. 2015. “Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates”. Plos One 10 (12): e0144769.

In die Zwischenablage einfügen

Wibberg, D., Rupp, O., Blom, J., Jelonek, L., Kröber, M., Verwaaijen, B., Goesmann, A., Albaum, S., Grosch, R., Pühler, A., et al. (2015). Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates. Plos One 10:e0144769.

In die Zwischenablage einfügen

Wibberg, D., et al., 2015. Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates. Plos One, 10(12): e0144769.

In die Zwischenablage einfügen

D. Wibberg, et al., “Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates”, Plos One, vol. 10, 2015, : e0144769.

In die Zwischenablage einfügen

Wibberg, D., Rupp, O., Blom, J., Jelonek, L., Kröber, M., Verwaaijen, B., Goesmann, A., Albaum, S., Grosch, R., Pühler, A., Schlüter, A.: Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates. Plos One. 10, : e0144769 (2015).

In die Zwischenablage einfügen

Wibberg, Daniel, Rupp, Oliver, Blom, Jochen, Jelonek, Lukas, Kröber, Magdalena, Verwaaijen, Bart, Goesmann, Alexander, Albaum, Stefan, Grosch, Rita, Pühler, Alfred, and Schlüter, Andreas. “Development of a Rhizoctonia solani AG1-IB Specific Gene Model Enables Comparative Genome Analyses between Phytopathogenic R-solani AG1-IA, AG1-IB, AG3 and AG8 Isolates”. Plos One 10.12 (2015): e0144769.

AMA

APA (6th ed.)

Chicago

Frontiers

Harvard

IEEE

LNCS

MLA