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Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV

Autor(en)
Bartholomaeus, Anika ; Wibberg, Daniel ; Winkler, Anika ; Pühler, Alfred ; Schlüter, Andreas ; Varrelmann, Mark
Normierter Zeitschriftentitel
PLOS ONE 
DOI
10.1371/journal.pone.0165965
ISSN
1932-6203
PubMed-ID
27814394
URL
https://pub.uni-bielefeld.de/record/2907431
Web of Science-ID
000386911100023

Weitere Details

Sprache
englisch
Darstellungsform
Text
Band
11
Artikelnummer
e0165965
Ausgabe
11

Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNApolymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani.

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