Journalartikel - Artikel | 2013 | Peer reviewed

Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons

Autor(en)

Klenke, Christin ; Widera, Darius ; Engelen, Thomas ; Müller, Janine ; Noll, Thomas

; Niehaus, Karsten ; Schmitz, M. ; Kaltschmidt, Barbara ; Kaltschmidt, Christian

Normierter Zeitschriftentitel

PLOS ONE

DOI

10.1371/journal.pone.0065280

PubMed-ID

23762333

URN

urn:nbn:de:0070-pub-25772653

URL

https://pub.uni-bielefeld.de/record/2577265

Web of Science-ID

000321094800033

Weitere Details

Sprache

englisch

Darstellungsform

Text

Band

Artikelnummer

e65280

Ausgabe

Link zum Volltext (intern)

https://pub.uni-bielefeld.de/record/2577265

DDC

570

Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.

Zitierstile

Klenke C, Widera D, Engelen T, et al. Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE. 2013;8(6): e65280.

In die Zwischenablage einfügen

Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., et al. (2013). Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE, 8(6), e65280. doi:10.1371/journal.pone.0065280

In die Zwischenablage einfügen

Klenke, Christin, Widera, Darius, Engelen, Thomas, Müller, Janine, Noll, Thomas, Niehaus, Karsten, Schmitz, M., Kaltschmidt, Barbara, and Kaltschmidt, Christian. 2013. “Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons”. PLoS ONE 8 (6): e65280.

In die Zwischenablage einfügen

Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., Kaltschmidt, B., and Kaltschmidt, C. (2013). Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE 8:e65280.

In die Zwischenablage einfügen

Klenke, C., et al., 2013. Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE, 8(6): e65280.

In die Zwischenablage einfügen

C. Klenke, et al., “Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons”, PLoS ONE, vol. 8, 2013, : e65280.

In die Zwischenablage einfügen

Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., Kaltschmidt, B., Kaltschmidt, C.: Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE. 8, : e65280 (2013).

In die Zwischenablage einfügen

Klenke, Christin, Widera, Darius, Engelen, Thomas, Müller, Janine, Noll, Thomas, Niehaus, Karsten, Schmitz, M., Kaltschmidt, Barbara, and Kaltschmidt, Christian. “Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons”. PLoS ONE 8.6 (2013): e65280.

In die Zwischenablage einfügen

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