Journalartikel - Artikel | | Peer reviewed Open Access

Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.

Autor(en)
Sporbert, Anje ; Cseresnyes, Zoltan ; Heidbreder, Meike ; Domaing, Petra ; Hauser, Stefan ; Kaltschmidt, Barbara ; Kaltschmidt, Christian ; Heilemann, Mike ; Widera, Darius
Normierter Zeitschriftentitel
PLOS ONE 
DOI
10.1371/journal.pone.0064023
PubMed-ID
23700448
URN
urn:nbn:de:0070-pub-25748661
URL
https://pub.uni-bielefeld.de/record/2574866
Web of Science-ID
000319330200106

This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.

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