Journalartikel - Artikel | 2014 | Peer reviewed

Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans

Autor(en)

Wittmann, Johannes ; Dreiseikelmann, Brigitte ; Rohde, Christine ; Rohde, Manfred ; Sikorski, Johannes

Normierter Zeitschriftentitel

PLOS ONE

DOI

10.1371/journal.pone.0086935

ISSN

1932-6203

PubMed-ID

24466294

URL

https://pub.uni-bielefeld.de/record/2664342

Web of Science-ID

000330283100235

Weitere Details

Sprache

englisch

Darstellungsform

Text

Band

Artikelnummer

e86935

Ausgabe

The clinical relevance of nosocomially acquired infections caused by multi-resistant Achromobacter strains is rapidly increasing. Here, a diverse set of 61 Achromobacter xylosoxidans strains was characterized by MultiLocus Sequence Typing and Phenotype MicroArray technology. The strains were further analyzed in regard to their susceptibility to 35 antibiotics and to 34 different and newly isolated bacteriophages from the environment. A large proportion of strains were resistant against numerous antibiotics such as cephalosporines, aminoglycosides and quinolones, whereas piperacillin-tazobactam, ticarcillin, mezlocillin and imipenem were still inhibitory. We also present the first expanded study on bacteriophages of the genus Achromobacter that has been so far a blank slate with respect to phage research. The phages were isolated mainly from several waste water treatment plants in Germany. Morphological analysis of all of these phages by electron microscopy revealed a broad diversity with different members of the order Caudovirales, including the families Siphoviridae, Myoviridae, and Podoviridae. A broad spectrum of different host ranges could be determined for several phages that lysed up to 24 different and in part highly antibiotic resistant strains. Molecular characterisation by DNA restriction analysis revealed that all phages contain linear double-stranded DNA. Their restriction patterns display distinct differences underlining their broad diversity.

Zitierstile

Wittmann J, Dreiseikelmann B, Rohde C, Rohde M, Sikorski J. Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans. PLoS ONE. 2014;9(1): e86935.

In die Zwischenablage einfügen

Wittmann, J., Dreiseikelmann, B., Rohde, C., Rohde, M., & Sikorski, J. (2014). Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans. PLoS ONE, 9(1), e86935. doi:10.1371/journal.pone.0086935

In die Zwischenablage einfügen

Wittmann, Johannes, Dreiseikelmann, Brigitte, Rohde, Christine, Rohde, Manfred, and Sikorski, Johannes. 2014. “Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans”. PLoS ONE 9 (1): e86935.

In die Zwischenablage einfügen

Wittmann, J., Dreiseikelmann, B., Rohde, C., Rohde, M., and Sikorski, J. (2014). Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans. PLoS ONE 9:e86935.

In die Zwischenablage einfügen

Wittmann, J., et al., 2014. Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans. PLoS ONE, 9(1): e86935.

In die Zwischenablage einfügen

J. Wittmann, et al., “Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans”, PLoS ONE, vol. 9, 2014, : e86935.

In die Zwischenablage einfügen

Wittmann, J., Dreiseikelmann, B., Rohde, C., Rohde, M., Sikorski, J.: Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans. PLoS ONE. 9, : e86935 (2014).

In die Zwischenablage einfügen

Wittmann, Johannes, Dreiseikelmann, Brigitte, Rohde, Christine, Rohde, Manfred, and Sikorski, Johannes. “Isolation and Characterization of Numerous Novel Phages Targeting Diverse Strains of the Ubiquitous and Opportunistic Pathogen Achromobacter xylosoxidans”. PLoS ONE 9.1 (2014): e86935.

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