Journalartikel - Artikel | | Peer reviewed

Genomics of high molecular weight plasmids isolated from an on-farm biopurification system

Autor(en)
Martini, Maria C. ; Wibberg, Daniel ; Lozano, Mauricio ; Torres Tejerizo, Gonzalo ; Albicoro, Francisco J. ; Jaenicke, Sebastian ; van Elsas, Jan Dirk ; Petroni, Alejandro ; Garcillan-Barcia, M. Pilar ; de la Cruz, Fernando ; Schlüter, Andreas ; Pühler, Alfred ; Pistorio, Mariano ; Lagares, Antonio ; Del Papa, Maria F.
Normierter Zeitschriftentitel
Scientific reports 
DOI
10.1038/srep28284
ISSN
2045-2322
PubMed-ID
27321040
URL
https://pub.uni-bielefeld.de/record/2904691
Web of Science-ID
000378270900001

Weitere Details

Sprache
englisch
Darstellungsform
Text
Band
6
Artikelnummer
28284
Ausgabe
1

The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.

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